About Louis

 

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Name:                      Louis Yu Pan Luk

Mobile:                    +44 29 2251 0161

Address:                  Room 2.73, School of Chemistry, Cardiff University, Main Building,        Park Place, Cardiff, CF10 3AT

E-mail:                     LukLY@cardiff.ac.uk

Hyperlink:              Google Scholar and ORCID

Education

2004-2010:   PhD, University of British Columbia

Mechanistic Studies on (S)-Norcoclaurine Synthase and Dimethylallyltryptophan Synthase

Principal Investigator: Professor Martin E. Tanner

1999-2004:   Chemistry and Microbiology & Immunology, University of British Columbia

Positions

2012-2015    Research Associate, School of Chemistry, Cardiff University, Cardiff, U.K. PI: Professor Rudolf K. Allemann

2010-2011    Postdoctoral Scholar, Department of Molecular Biology, University of Chicago, Chicago, U.S.A. PI: Professor Stephen B. H. Kent

2004-2010    Ph. D. Candidate, Department of Chemistry, University of British Columbia, Vancouver, Canada. PI: Professor Martin E. Tanner

2005-2009    Teaching Assistant/Tutor, Department of Chemistry, University of British Columbia, Vancouver, Canada. Supervisor: Dr Dana Zendrowski

2003-2003    NSERC USRA Scholar, Department of Microbiology & Immunology, University of British Columbia, Vancouver, Canada. PI: Professor Lindsay D. Eltis

Award Grants

Royal Society International Exchange Scheme. £10150. 03/2018-02/2020. Principal Investigator. Protein Nanoreactor: Encapsulating Toxic Biocatalyst for the Production of High-Value Cyclic Peptides.

Royal Society Research Grant. £15,000. 03/2018-02/2019. Principal Investigator. Large scale fermentation of cyclic proteins.

Leverhulme Trust. £176,847. 11/2017-10/2020. Principal Investigator. Designing Multifunctional Organocatalytic Artificial Enzymes.

Wellcome Seed Trust. £100,000. 08/2016-08/2018. Principal Investigator. Dihydrofolate reductase interactome: an unrecognised network that can control DNA synthesis and cell replication.

BBSRC. £402,824. 08/2014-07/2017. Co-investigator. Reaction-coupled dynamics in DHFR catalysis.

BBSRC. £510,222. 04/2017-03/2020. Co-investigator. Traceless, non-invasive and spatiotemporal control of protein activity in cells.

Competitive Awards and Honours

2006             UBC Graduate Fellowship

2005             Natural Sciences and Engineering Research Council (NSERC) Canada Graduate Scholarship Master (CGS M)

2005             Dr F. J. Nicholson Scholarship

2004-2006    Gladys Estella Laird Research Fellowship

2004             Graduate Entrance Scholarship

2002-2004    Dean’s Honour List

2003             NSERC Undergraduate Student Research Award

2002-2004    UBC Undergraduate Scholar Program Scholarship

2002             J. Fred Muir Memorial Scholarship in Science

Publications

  1. Świderek, K.; Nödling, A.; Tsai, Y-H.; Luk, L.Y.P.*; Moliner, V.* The Reaction Mechanism of Organocatalytic Michael Addition of Nitromethane to Cinnamaldehyde: A Case Study on Catalyst Regeneration and Solvent Effect. J. Phys. Chem. A. accepted. 
  2. Liao, J.H.; Tsai, C-H.; Patel, S.G.; Yang, J.T.; Tu, I-F.; Cicero, M.L.; Lipka-Lloyd M.; Wu, W-L.; Shen, W-J.; Ho, M-R.; Chou, C-C.; Sharma, G.R. Okanishi, H.; Luk, L.Y.P.; Tsai, Y-H.; Wu, S-H. Acetylome of Acinetobacter baumannii SK17 a highly conversed modification of histone-like protein HU. Front. Mol. Biosci. 4, 1-12 (2017). DOI: 10.3389/fmolb.201700077
  3. Angelastro, A.; Dawson, W.D.; Luk, L.Y.P.; Loveridge, E.J.; Allemann, R.K. Chemoenzymatic Assembly of Isotopically Labeled Folates. J. Am. Chem. Soc.139, 13047–13054 (2017). DOI: 10.1021/jacs.7b06358
  4. S.-J. Lai, I-F. Tu, W.-L. Wu, J.-T. Yang, L. Y. P. Luk, M.-C. Lai, Y.-H. Tsai, S.-H. Wu, Site-specific His/Asp phosphoproteomic analysis of prokaryotes reveals putative targets for drug resistance. BMC Microbiol. 
  5. Scott, A.F.; Luk, L.Y.P.; Allemann, R.K. Chemical ligation and isotope labeling to locate dynamic effects. Methods in Enzymology. 596, 23-41 (2017). DOI: 10.1016/bs.mie.2017.06.040
  6. , R. L,; , R.L.; , A.;  , G.;  
  7. Angelastro, A.; 
  8. Castillo, J.P.; Sánchez-Rodríguez, J.E.; Hyde, H.C.; Zaelzer, C.A.; Aguayo, D.; Sepúlveda, R.V.; Luk, L.Y.P.; Kent, S.B.H.; Gonzalez-Nilo, F.D.; Bezanilla, F.; Latorre, R.β1-subunit–induced structural rearrangements of the Ca2+– and voltage-activated K+ (BK) channelProc. Natl. Acad. Sci. 113, E3231–E3239, (2016) DOI: 10.1073/pnas.1606381113
  9. Ruiz-Perniaa, J.J.; Behiry, E.; Luk, L.Y.P.; Loveridge, E.J., Tuñónc, I., Moliner, V.; Allemann, R.K.; Minimization of Dynamic Effects in the Evolution of Dihydrofolate Reductase, Chem. Sci. 7, 3248-3255 (2015) DOI: 10.1039/C5SC04209G
  10. Luk, L.Y.P.; Ruiz-Pernia, J.J.; Adesina, A.S.; Loveridge, E.J.; Tuñón, I.; Moliner, V.; Allemann, R.K.; Chemical ligation and isotope labeling to locate dynamic effects during catalysis by dihydrofolate reductase, Angew. Chem. Int. Ed., 54, 9016-9020 (2015) DOI: 10.1002/anie.201503968
  11. Luk, L.Y.P.; Loveridge, E.J.; Allemann, R.K.; Protein motions, dynamic effects and thermal stability in dihydrofolate reductase from the hyperthermophile Thermotoga maritima, book chapter, pp 99-113, Springer.
  12. Luk, L.Y.P.; Loveridge, E.J.; Allemann, R.K.; Protein motions and dynamic effects in enzyme catalysis, Phys. Chem. Chem. Phys., 17, 30817-30827 (2015) DOI: 10.1039/c5cp00794a.
  13. Luk, L.Y.P.; Ruiz-Pernía, J.J.; Dawson, W.M.; Loveridge, E.J.; Tuñón, I.; Moliner, V.; Allemann, R.K.; Protein isotope effects in dihydrofolate reductase from Geobacillus stearothermophilus show entropic-enthalpic compensatory effects on the rate constant, J. Am. Chem. Soc. 136, 17317-17323 (2014) DOI: 10.1021/ja5102536
  14. Guo, J.; Loveridge, E.J.; Luk, L.Y.P.; Allemann, R.K.; Thermal adaptation of dihydrofolate reductase from the moderate thermophile Geobacillus stearothermophilus, Biochemistry, 53, 2855-2863 (2014) DOI: 1021/bi500238q
  15. Behiry, E.M.; Luk, L.Y.P.; Matthews, S.M.; Loveridge, E.J.; Allemann, R.K.; Role of the occluded conformation in bacterial dihydrofolate reductases, Biochemistry, 53, 4761-4768 (2014) DOI: 1021/bi508507v
  16. Luk, L.Y.P.; Loveridge, E.J.; Allemann, R.K.; Different dynamical effects in mesophilic and hyperthermophilic dihydrofolate reductases, J. Am. Chem. Soc., 136, 6862-6865 (2014) DOI: 10.1021/ja502673h
  17. Luk, L.Y.P.; Ruiz-Pernia, J.J.; García-Meseguer, R.; Marti, S.; Loveridge, E.J.; Tuñón, I.; Moliner, V.; Allemann, R.K.; Increased dynamic effects in a ‘dynamic knockout’ of dihydrofolate reductase from Escherichia coli, J. Am. Chem. Soc., 135, 18689-18696 (2013) DOI: 10.1021/ja410519h
  18. Luk, L.Y.P.; Ruiz-Pernia, J.J.; Dawson, W.M.; Roca, M.; Loveridge, E.J.; Glowacki, D.R.; Harvey, J.N.; Mulholland, A.J.; Tuñón, I.; Moliner, V.; Allemann, R.K.; Unravelling the role of protein dynamics in dihydrofolate reductase catalysis, Proc. Natl. Acad. Sci., 110, 16344-16349 (2013) DOI: 10.1073/PNAS.1312437110
  19. Guo, J.; Loveridge, E.J.; Luk, L.Y.P.; Allemann, R.K.; Effect of dimerization on catalysis by dihydrofolate reductase, Biochemistry, 52, 3881-3887 (2013) DOI: 10.1021/bi4005073
  20. Mahmoodi, N.; Qi, Q.; Luk, L.Y.P.; Tanner, M.E.; Rearrangements in the mechanisms of the indole alkaloid prenyltransferases, Pure Appl. Chem., 85, 1935-1948 (2013). DOI: 10.1351/PAC-CON-13-02-02
  21. Luk, L.Y.P.; Qian, Q.; Tanner, M.E.; A Cope rearrangement in the reaction catalyzed by dimethylallyltryptophan synthase? J. Am. Chem. Soc. 133, 12342-12345 (2011); DOI: 10.1021/ja2034969
  22. Luk, L.Y.P.; Tanner, M.E.; Mechanism of dimethylallyltryptophan synthase: evidence for a dimethylallyl cation intermediate in an aromatic prenyltransferase reaction, J. Am. Chem. Soc. 131, 13932-13933 (2009); DOI: 10.1021/ja906485u
  23. Luk, L.Y.P.; Bunn, S.; Liscombe, D.K.; Facchini, P.J.; Tanner, M.E.; Mechanistic studies on norcoclaurine synthase of benzylisoquinoline alkaloid biosynthesis: an enzymatic Pictet-Spengler reaction, Biochemistry, 46, 10153-10161 (2007); DOI: 10.1021/bi700752n

Academic Conference Presentations

Enabling organocatalysis in a genetically coded protein environment. Nachwuchswissenschaftler-Symposium Bioorganische Chemie, Berlin, Germany, 2017

The origin of dynamic effects in enzyme transition state. Telluride Protein Dynamics Workshop, Colorado, the US, 2015

Locating dynamic effects by studying EcDHFR isotopic hybrids. International Cofactor Conference, Parma, Italy, 2014

Mechanistic studies on dimethylallyltryptophan synthase. Volcano Conference, Washington, the US, 2010

Mechanistic studies on dimethylallyltryptophan synthase supports a Dissociative Pathway. Gordon Conference: Enzymes, Coenzymes & Metabolic Pathways, New Hampshire, 2009

Mechanistic studies on dimethylallyltryptophan synthase. Volcano Conference, Washington, 2009

Mechanistic studies on (S)-norcoclaurine synthase. Gordon Conference: Bioorganic Chemistry, New Hampshire, 2008

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